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dcas9 krab fusion protein  (Addgene inc)


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    Addgene inc dcas9 krab fusion protein
    Dcas9 Krab Fusion Protein, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 206 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dcas9+krab+fusion+protein/pLV+hU6-sgRNA+hUbC-dCas9-KRAB-T2a-Puro+(Plasmid+%2371236)/pm41203724-154-13-16
    Average 96 stars, based on 206 article reviews
    dcas9 krab fusion protein - by Bioz Stars, 2026-10
    96/100 stars

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    Expressing:

    Article Title: Systematic identification of post-transcriptional regulatory modules
    Article Snippet: Samples were sequenced using paired-end 150 bp sequencing on an Illumina MiSeq sequencer. .. K562 cells (ATCC CCL-243) expressing dCas9-KRAB fusion protein were constructed by lentiviral delivery of pMH0006 (Addgene #135448) and FACS isolation of BFP-positive cells. .. The lentiviral constructs were co-transfected with pCMV-dR8.91 (Andwin Scientific NC2092494) and pMD2.D (Addgene #12259) plasmids using TransIT-Lenti (Mirus 75814-982) into 293 T cells (ATCC CCL-3216), following the manufacturer’s protocol.

    Article Title: A systematic search for RNA structural switches across the human transcriptome.
    Article Snippet: .. CRISPRi-mediated and small interfering RNA-mediated gene knockdown Jurkat cells expressing the dCas9–KRAB fusion protein were constructed by lentiviral delivery of pMH0006 (Addgene, cat. no. 135448) and FACS isolation of BFP-positive cells. .. Guide RNA sequences for CRISPRi-mediated gene knockdown were cloned into pCRISPRia-v2 (Addgene, cat. no. 84832) via BstXI-BlpI sites.

    Article Title: A systematic search for RNA structural switches across the human transcriptome
    Article Snippet: .. Jurkat cells expressing the dCas9–KRAB fusion protein were constructed by lentiviral delivery of pMH0006 (Addgene, cat. no. 135448) and FACS isolation of BFP-positive cells. .. Guide RNA sequences for CRISPRi-mediated gene knockdown were cloned into pCRISPRia-v2 (Addgene, cat. no. 84832) via BstXI-BlpI sites.

    Article Title: Recurrent FBXW7 mutations bypass Wnt/β-catenin addiction in cancer
    Article Snippet: Lentiviruses and retroviruses were produced by co-transfecting the transfer plasmids with pMD2.G (Addgene #12259, a gift from Didier Trono) and psPAX2 (Addgene #12260, a gift from Didier Trono) or pCL-Eco (Addgene #12371, a gift from Inder Verma) into HEK293FT cells, as previously described . .. The transfer plasmids used in this study included FuCas9Cherry (Addgene #70182, a gift from Marco Herold) for constitutively expressing Cas9 protein, Lenti-dCas9-KRAB-blast (Addgene #89567, a gift from Gary Hon) for constitutively expressing dCas9-KRAB fusion protein, FgH1tUTG (Addgene #70183, a gift from Marco Herold) for expressing doxycycline (dox) inducible sgRNA, pHR’CMV-FBXW7 R465C (a gift from Patrick Tan) for expressing the mutant FBXW7, MSCV MYC T58A puro (Addgene #18773, a gift from Scott Lowe) for expressing the mutant MYC, and MIGR1-Cyclin E-AA (Addgene #47498, a gift from Alex Minella) for expressing the mutant Cyclin E. After transduction, cells were selected with the corresponding antibiotics or fluorescence-activated cell sorting (FACS). ..

    Article Title: Recurrent mutations in tumor suppressor FBXW7 bypass Wnt/β-catenin addiction in cancer
    Article Snippet: Lentiviruses and retroviruses were produced by cotransfecting the transfer plasmids with pMD2.G (Addgene, no. 12259, a gift from D. Trono) and psPAX2 (Addgene, no. 12260, a gift from D. Trono) or pCL-Eco (Addgene, no. 12371, a gift from I. Verma) ( ) into human embryonic kidney–293FT cells, as previously described ( ). .. The transfer plasmids used in this study included FuCas9Cherry (Addgene, no. 70182, a gift from M. Herold) ( ) for constitutively expressing Cas9 protein, Lenti-dCas9-KRAB-blast (Addgene, no. 89567, a gift from G. Hon) ( ) for constitutively expressing dCas9-KRAB fusion protein, FgH1tUTG (Addgene, no. 70183, a gift from M. Herold) ( ) for expressing dox-inducible sgRNA, pHR’CMV-FBXW7 R465C (a gift from P. Tan) ( ) for expressing the mutant FBXW7, MSCV MYC T58A puro (Addgene, no. 18773, a gift from S. Lowe) ( ) for expressing the mutant MYC, and MIGR1–Cyclin E–AA (Addgene, no. 47498, a gift from A. Minella) ( ) for expressing the mutant Cyclin E. After transduction, cells were selected with the corresponding antibiotics or fluorescence-activated cell sorting. ..

    Construct:

    Article Title: Systematic identification of post-transcriptional regulatory modules
    Article Snippet: Samples were sequenced using paired-end 150 bp sequencing on an Illumina MiSeq sequencer. .. K562 cells (ATCC CCL-243) expressing dCas9-KRAB fusion protein were constructed by lentiviral delivery of pMH0006 (Addgene #135448) and FACS isolation of BFP-positive cells. .. The lentiviral constructs were co-transfected with pCMV-dR8.91 (Andwin Scientific NC2092494) and pMD2.D (Addgene #12259) plasmids using TransIT-Lenti (Mirus 75814-982) into 293 T cells (ATCC CCL-3216), following the manufacturer’s protocol.

    Article Title: A systematic search for RNA structural switches across the human transcriptome.
    Article Snippet: .. CRISPRi-mediated and small interfering RNA-mediated gene knockdown Jurkat cells expressing the dCas9–KRAB fusion protein were constructed by lentiviral delivery of pMH0006 (Addgene, cat. no. 135448) and FACS isolation of BFP-positive cells. .. Guide RNA sequences for CRISPRi-mediated gene knockdown were cloned into pCRISPRia-v2 (Addgene, cat. no. 84832) via BstXI-BlpI sites.

    Article Title: Interrogating subclonal heterogeneity of liver cancer with single-cell multi-omics analysis.
    Article Snippet: .. These sgRNAs were then constructed into a lentiviral vector that concurrently expressed the dCas9-KRAB fusion protein (Addgene Cat. 71236). .. Post-infection, the cells were cultured for 15 days in DMEM (HyClone, Cat. SH30243) supplemented with 10% FBS (Sigma, Cat. F0193), 1% L-glutamine (Gibco, Cat. 35050-061), and 1% penicillin–streptomycin (Gibco, Cat. 15140-122).

    Article Title: A systematic search for RNA structural switches across the human transcriptome
    Article Snippet: .. Jurkat cells expressing the dCas9–KRAB fusion protein were constructed by lentiviral delivery of pMH0006 (Addgene, cat. no. 135448) and FACS isolation of BFP-positive cells. .. Guide RNA sequences for CRISPRi-mediated gene knockdown were cloned into pCRISPRia-v2 (Addgene, cat. no. 84832) via BstXI-BlpI sites.

    Article Title: Interrogating subclonal heterogeneity of liver cancer with single-cell multi-omics analysis
    Article Snippet: Post-infection, the cells were cultured for 15 days in DMEM (HyClone, Cat. SH30243) supplemented with 10% FBS (Sigma, Cat. F0193), 1% L-glutamine (Gibco, Cat. 35050-061), and 1% penicillin–streptomycin (Gibco, Cat. 15140-122). .. These sgRNAs were then constructed into a lentiviral vector that concurrently expressed the dCas9-KRAB fusion protein (Addgene Cat. 71236). .. These sgRNAs were then constructed into a lentiviral vector that concurrently expressed the dCas9-KRAB fusion protein (Addgene Cat. 71236).

    FACS:

    Article Title: Systematic identification of post-transcriptional regulatory modules
    Article Snippet: Samples were sequenced using paired-end 150 bp sequencing on an Illumina MiSeq sequencer. .. K562 cells (ATCC CCL-243) expressing dCas9-KRAB fusion protein were constructed by lentiviral delivery of pMH0006 (Addgene #135448) and FACS isolation of BFP-positive cells. .. The lentiviral constructs were co-transfected with pCMV-dR8.91 (Andwin Scientific NC2092494) and pMD2.D (Addgene #12259) plasmids using TransIT-Lenti (Mirus 75814-982) into 293 T cells (ATCC CCL-3216), following the manufacturer’s protocol.

    Article Title: A systematic search for RNA structural switches across the human transcriptome.
    Article Snippet: .. CRISPRi-mediated and small interfering RNA-mediated gene knockdown Jurkat cells expressing the dCas9–KRAB fusion protein were constructed by lentiviral delivery of pMH0006 (Addgene, cat. no. 135448) and FACS isolation of BFP-positive cells. .. Guide RNA sequences for CRISPRi-mediated gene knockdown were cloned into pCRISPRia-v2 (Addgene, cat. no. 84832) via BstXI-BlpI sites.

    Article Title: A systematic search for RNA structural switches across the human transcriptome
    Article Snippet: .. Jurkat cells expressing the dCas9–KRAB fusion protein were constructed by lentiviral delivery of pMH0006 (Addgene, cat. no. 135448) and FACS isolation of BFP-positive cells. .. Guide RNA sequences for CRISPRi-mediated gene knockdown were cloned into pCRISPRia-v2 (Addgene, cat. no. 84832) via BstXI-BlpI sites.

    Article Title: Recurrent FBXW7 mutations bypass Wnt/β-catenin addiction in cancer
    Article Snippet: Lentiviruses and retroviruses were produced by co-transfecting the transfer plasmids with pMD2.G (Addgene #12259, a gift from Didier Trono) and psPAX2 (Addgene #12260, a gift from Didier Trono) or pCL-Eco (Addgene #12371, a gift from Inder Verma) into HEK293FT cells, as previously described . .. The transfer plasmids used in this study included FuCas9Cherry (Addgene #70182, a gift from Marco Herold) for constitutively expressing Cas9 protein, Lenti-dCas9-KRAB-blast (Addgene #89567, a gift from Gary Hon) for constitutively expressing dCas9-KRAB fusion protein, FgH1tUTG (Addgene #70183, a gift from Marco Herold) for expressing doxycycline (dox) inducible sgRNA, pHR’CMV-FBXW7 R465C (a gift from Patrick Tan) for expressing the mutant FBXW7, MSCV MYC T58A puro (Addgene #18773, a gift from Scott Lowe) for expressing the mutant MYC, and MIGR1-Cyclin E-AA (Addgene #47498, a gift from Alex Minella) for expressing the mutant Cyclin E. After transduction, cells were selected with the corresponding antibiotics or fluorescence-activated cell sorting (FACS). ..

    Article Title: Recurrent mutations in tumor suppressor FBXW7 bypass Wnt/β-catenin addiction in cancer
    Article Snippet: Lentiviruses and retroviruses were produced by cotransfecting the transfer plasmids with pMD2.G (Addgene, no. 12259, a gift from D. Trono) and psPAX2 (Addgene, no. 12260, a gift from D. Trono) or pCL-Eco (Addgene, no. 12371, a gift from I. Verma) ( ) into human embryonic kidney–293FT cells, as previously described ( ). .. The transfer plasmids used in this study included FuCas9Cherry (Addgene, no. 70182, a gift from M. Herold) ( ) for constitutively expressing Cas9 protein, Lenti-dCas9-KRAB-blast (Addgene, no. 89567, a gift from G. Hon) ( ) for constitutively expressing dCas9-KRAB fusion protein, FgH1tUTG (Addgene, no. 70183, a gift from M. Herold) ( ) for expressing dox-inducible sgRNA, pHR’CMV-FBXW7 R465C (a gift from P. Tan) ( ) for expressing the mutant FBXW7, MSCV MYC T58A puro (Addgene, no. 18773, a gift from S. Lowe) ( ) for expressing the mutant MYC, and MIGR1–Cyclin E–AA (Addgene, no. 47498, a gift from A. Minella) ( ) for expressing the mutant Cyclin E. After transduction, cells were selected with the corresponding antibiotics or fluorescence-activated cell sorting. ..

    Isolation:

    Article Title: Systematic identification of post-transcriptional regulatory modules
    Article Snippet: Samples were sequenced using paired-end 150 bp sequencing on an Illumina MiSeq sequencer. .. K562 cells (ATCC CCL-243) expressing dCas9-KRAB fusion protein were constructed by lentiviral delivery of pMH0006 (Addgene #135448) and FACS isolation of BFP-positive cells. .. The lentiviral constructs were co-transfected with pCMV-dR8.91 (Andwin Scientific NC2092494) and pMD2.D (Addgene #12259) plasmids using TransIT-Lenti (Mirus 75814-982) into 293 T cells (ATCC CCL-3216), following the manufacturer’s protocol.

    Article Title: A systematic search for RNA structural switches across the human transcriptome.
    Article Snippet: .. CRISPRi-mediated and small interfering RNA-mediated gene knockdown Jurkat cells expressing the dCas9–KRAB fusion protein were constructed by lentiviral delivery of pMH0006 (Addgene, cat. no. 135448) and FACS isolation of BFP-positive cells. .. Guide RNA sequences for CRISPRi-mediated gene knockdown were cloned into pCRISPRia-v2 (Addgene, cat. no. 84832) via BstXI-BlpI sites.

    Article Title: A systematic search for RNA structural switches across the human transcriptome
    Article Snippet: .. Jurkat cells expressing the dCas9–KRAB fusion protein were constructed by lentiviral delivery of pMH0006 (Addgene, cat. no. 135448) and FACS isolation of BFP-positive cells. .. Guide RNA sequences for CRISPRi-mediated gene knockdown were cloned into pCRISPRia-v2 (Addgene, cat. no. 84832) via BstXI-BlpI sites.

    Knockdown:

    Article Title: A systematic search for RNA structural switches across the human transcriptome.
    Article Snippet: .. CRISPRi-mediated and small interfering RNA-mediated gene knockdown Jurkat cells expressing the dCas9–KRAB fusion protein were constructed by lentiviral delivery of pMH0006 (Addgene, cat. no. 135448) and FACS isolation of BFP-positive cells. .. Guide RNA sequences for CRISPRi-mediated gene knockdown were cloned into pCRISPRia-v2 (Addgene, cat. no. 84832) via BstXI-BlpI sites.

    Plasmid Preparation:

    Article Title: Interrogating subclonal heterogeneity of liver cancer with single-cell multi-omics analysis.
    Article Snippet: .. These sgRNAs were then constructed into a lentiviral vector that concurrently expressed the dCas9-KRAB fusion protein (Addgene Cat. 71236). .. Post-infection, the cells were cultured for 15 days in DMEM (HyClone, Cat. SH30243) supplemented with 10% FBS (Sigma, Cat. F0193), 1% L-glutamine (Gibco, Cat. 35050-061), and 1% penicillin–streptomycin (Gibco, Cat. 15140-122).

    Article Title: Interrogating subclonal heterogeneity of liver cancer with single-cell multi-omics analysis
    Article Snippet: Post-infection, the cells were cultured for 15 days in DMEM (HyClone, Cat. SH30243) supplemented with 10% FBS (Sigma, Cat. F0193), 1% L-glutamine (Gibco, Cat. 35050-061), and 1% penicillin–streptomycin (Gibco, Cat. 15140-122). .. These sgRNAs were then constructed into a lentiviral vector that concurrently expressed the dCas9-KRAB fusion protein (Addgene Cat. 71236). .. These sgRNAs were then constructed into a lentiviral vector that concurrently expressed the dCas9-KRAB fusion protein (Addgene Cat. 71236).

    Mutagenesis:

    Article Title: Recurrent FBXW7 mutations bypass Wnt/β-catenin addiction in cancer
    Article Snippet: Lentiviruses and retroviruses were produced by co-transfecting the transfer plasmids with pMD2.G (Addgene #12259, a gift from Didier Trono) and psPAX2 (Addgene #12260, a gift from Didier Trono) or pCL-Eco (Addgene #12371, a gift from Inder Verma) into HEK293FT cells, as previously described . .. The transfer plasmids used in this study included FuCas9Cherry (Addgene #70182, a gift from Marco Herold) for constitutively expressing Cas9 protein, Lenti-dCas9-KRAB-blast (Addgene #89567, a gift from Gary Hon) for constitutively expressing dCas9-KRAB fusion protein, FgH1tUTG (Addgene #70183, a gift from Marco Herold) for expressing doxycycline (dox) inducible sgRNA, pHR’CMV-FBXW7 R465C (a gift from Patrick Tan) for expressing the mutant FBXW7, MSCV MYC T58A puro (Addgene #18773, a gift from Scott Lowe) for expressing the mutant MYC, and MIGR1-Cyclin E-AA (Addgene #47498, a gift from Alex Minella) for expressing the mutant Cyclin E. After transduction, cells were selected with the corresponding antibiotics or fluorescence-activated cell sorting (FACS). ..

    Article Title: Recurrent mutations in tumor suppressor FBXW7 bypass Wnt/β-catenin addiction in cancer
    Article Snippet: Lentiviruses and retroviruses were produced by cotransfecting the transfer plasmids with pMD2.G (Addgene, no. 12259, a gift from D. Trono) and psPAX2 (Addgene, no. 12260, a gift from D. Trono) or pCL-Eco (Addgene, no. 12371, a gift from I. Verma) ( ) into human embryonic kidney–293FT cells, as previously described ( ). .. The transfer plasmids used in this study included FuCas9Cherry (Addgene, no. 70182, a gift from M. Herold) ( ) for constitutively expressing Cas9 protein, Lenti-dCas9-KRAB-blast (Addgene, no. 89567, a gift from G. Hon) ( ) for constitutively expressing dCas9-KRAB fusion protein, FgH1tUTG (Addgene, no. 70183, a gift from M. Herold) ( ) for expressing dox-inducible sgRNA, pHR’CMV-FBXW7 R465C (a gift from P. Tan) ( ) for expressing the mutant FBXW7, MSCV MYC T58A puro (Addgene, no. 18773, a gift from S. Lowe) ( ) for expressing the mutant MYC, and MIGR1–Cyclin E–AA (Addgene, no. 47498, a gift from A. Minella) ( ) for expressing the mutant Cyclin E. After transduction, cells were selected with the corresponding antibiotics or fluorescence-activated cell sorting. ..

    Transduction:

    Article Title: Recurrent FBXW7 mutations bypass Wnt/β-catenin addiction in cancer
    Article Snippet: Lentiviruses and retroviruses were produced by co-transfecting the transfer plasmids with pMD2.G (Addgene #12259, a gift from Didier Trono) and psPAX2 (Addgene #12260, a gift from Didier Trono) or pCL-Eco (Addgene #12371, a gift from Inder Verma) into HEK293FT cells, as previously described . .. The transfer plasmids used in this study included FuCas9Cherry (Addgene #70182, a gift from Marco Herold) for constitutively expressing Cas9 protein, Lenti-dCas9-KRAB-blast (Addgene #89567, a gift from Gary Hon) for constitutively expressing dCas9-KRAB fusion protein, FgH1tUTG (Addgene #70183, a gift from Marco Herold) for expressing doxycycline (dox) inducible sgRNA, pHR’CMV-FBXW7 R465C (a gift from Patrick Tan) for expressing the mutant FBXW7, MSCV MYC T58A puro (Addgene #18773, a gift from Scott Lowe) for expressing the mutant MYC, and MIGR1-Cyclin E-AA (Addgene #47498, a gift from Alex Minella) for expressing the mutant Cyclin E. After transduction, cells were selected with the corresponding antibiotics or fluorescence-activated cell sorting (FACS). ..

    Article Title: Recurrent mutations in tumor suppressor FBXW7 bypass Wnt/β-catenin addiction in cancer
    Article Snippet: Lentiviruses and retroviruses were produced by cotransfecting the transfer plasmids with pMD2.G (Addgene, no. 12259, a gift from D. Trono) and psPAX2 (Addgene, no. 12260, a gift from D. Trono) or pCL-Eco (Addgene, no. 12371, a gift from I. Verma) ( ) into human embryonic kidney–293FT cells, as previously described ( ). .. The transfer plasmids used in this study included FuCas9Cherry (Addgene, no. 70182, a gift from M. Herold) ( ) for constitutively expressing Cas9 protein, Lenti-dCas9-KRAB-blast (Addgene, no. 89567, a gift from G. Hon) ( ) for constitutively expressing dCas9-KRAB fusion protein, FgH1tUTG (Addgene, no. 70183, a gift from M. Herold) ( ) for expressing dox-inducible sgRNA, pHR’CMV-FBXW7 R465C (a gift from P. Tan) ( ) for expressing the mutant FBXW7, MSCV MYC T58A puro (Addgene, no. 18773, a gift from S. Lowe) ( ) for expressing the mutant MYC, and MIGR1–Cyclin E–AA (Addgene, no. 47498, a gift from A. Minella) ( ) for expressing the mutant Cyclin E. After transduction, cells were selected with the corresponding antibiotics or fluorescence-activated cell sorting. ..

    Fluorescence:

    Article Title: Recurrent FBXW7 mutations bypass Wnt/β-catenin addiction in cancer
    Article Snippet: Lentiviruses and retroviruses were produced by co-transfecting the transfer plasmids with pMD2.G (Addgene #12259, a gift from Didier Trono) and psPAX2 (Addgene #12260, a gift from Didier Trono) or pCL-Eco (Addgene #12371, a gift from Inder Verma) into HEK293FT cells, as previously described . .. The transfer plasmids used in this study included FuCas9Cherry (Addgene #70182, a gift from Marco Herold) for constitutively expressing Cas9 protein, Lenti-dCas9-KRAB-blast (Addgene #89567, a gift from Gary Hon) for constitutively expressing dCas9-KRAB fusion protein, FgH1tUTG (Addgene #70183, a gift from Marco Herold) for expressing doxycycline (dox) inducible sgRNA, pHR’CMV-FBXW7 R465C (a gift from Patrick Tan) for expressing the mutant FBXW7, MSCV MYC T58A puro (Addgene #18773, a gift from Scott Lowe) for expressing the mutant MYC, and MIGR1-Cyclin E-AA (Addgene #47498, a gift from Alex Minella) for expressing the mutant Cyclin E. After transduction, cells were selected with the corresponding antibiotics or fluorescence-activated cell sorting (FACS). ..

    Article Title: Recurrent mutations in tumor suppressor FBXW7 bypass Wnt/β-catenin addiction in cancer
    Article Snippet: Lentiviruses and retroviruses were produced by cotransfecting the transfer plasmids with pMD2.G (Addgene, no. 12259, a gift from D. Trono) and psPAX2 (Addgene, no. 12260, a gift from D. Trono) or pCL-Eco (Addgene, no. 12371, a gift from I. Verma) ( ) into human embryonic kidney–293FT cells, as previously described ( ). .. The transfer plasmids used in this study included FuCas9Cherry (Addgene, no. 70182, a gift from M. Herold) ( ) for constitutively expressing Cas9 protein, Lenti-dCas9-KRAB-blast (Addgene, no. 89567, a gift from G. Hon) ( ) for constitutively expressing dCas9-KRAB fusion protein, FgH1tUTG (Addgene, no. 70183, a gift from M. Herold) ( ) for expressing dox-inducible sgRNA, pHR’CMV-FBXW7 R465C (a gift from P. Tan) ( ) for expressing the mutant FBXW7, MSCV MYC T58A puro (Addgene, no. 18773, a gift from S. Lowe) ( ) for expressing the mutant MYC, and MIGR1–Cyclin E–AA (Addgene, no. 47498, a gift from A. Minella) ( ) for expressing the mutant Cyclin E. After transduction, cells were selected with the corresponding antibiotics or fluorescence-activated cell sorting. ..

    other:

    Article Title: Systematic identification of post-transcriptional regulatory modules.
    Article Snippet: Samples were sequenced using paired-end 150 bp sequencing on an Illumina MiSeq sequencer.



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